New Methods for Isolation of Pathogenic Bacteria from Milk

New methods of pathogenic bacteria elimination.

The growing bacterial resistance to antibiotics calls for the elaboration of new pathogens elimination strategies. Some of these methods are based on the conjugative transfer of recombinant plasmids able to eliminate pathogenic recipients by plasmid run-away replication or by killing activity of plasmid-encoded bacteriocins. Using live bacteria as donors of plasmid vectors carrying killing dete...

Isolation and Identification of Pathogenic Bacteria from Drinking Water

Drinking water microbiological quality was primarily determined by enumeration of “indicator organisms”, whose presence indicates faecal contamination. The presence of the indicators is often a key in assessing potential public health risks due to bacterial pathogens and is used in drinking water quality regulations and guidelines in many countries. The enumeration of fecal coliform specificall...

Isolation of mammalian pathogenic bacteria using silkworms.

We developed a method to predict bacterial pathogenicity against mammals by measuring bacterial virulence in silkworms at 37°C, human body temperature. One hundred and twenty-two strains of bacteria were isolated from the intestines of fish and shellfish and tested for their virulence against silkworms. Overnight cultures of 50 strains killed at least 50% of the silkworms when injected into the...

Isolation and Characterization of Antibiotics Resistant Bacteria from Raw Milk

Objective: Isolation and identification of antibiotics resistant bacteria in raw milk. Methods: Luria-Bertani plates containing ampicillin (32 μg/mL), clindamycin (4 μg/mL), or chloramphenicol (32 μg/mL) were used in isolating the antibiotics resistant bacteria from samples; 16S rRNA analysis method was used to identify the isolates; blood agar plates were used in hemolysis assay; K-B disk diff...

Methods of Isolation of Bacteria